Morgan Emtner1, John T. Brandt, Ulrika Johansson, B Jouper, Linda Fryklund, P. Roos
1Department of Biochemistry, University of Uppsala, Sweden
Tóm tắt
ABSTRACT
Affinity-purified lactogenic receptors from female rat liver microsomal membranes were used to raise antibodies in female Balb/c mice. Mouse spleen and myeloma cells were fused and hybridoma-derived monoclonal antibodies (Mabs) were produced by in-vitro cell culture. Mab from a selected clone was sequentially purified by chromatography on a thiophilic gel and on agarose-bound protein A. The Mab was found to be of IgG1 subclass and of κ type.
The Mab recognized membrane-bound and solubilized (by the detergent heptaoxyethylene lauryl ether; G3707) receptors as well as receptors purified by affinity chromatography and subsequent sodium dodecyl sulphate (SDS) electrophoresis from female rat liver. The Mab bound to receptors from several other female rat tissues, such as ovary, kidney and adrenal, whereas there was no binding to liver receptors from cow and rabbit. Displacement experiments showed that the Mab was specific for a lactogenic type of receptor, in agreement with the finding that the Mab did not recognize receptors from male rat liver. The Mab also bound to cytoplasmic receptors (present in the supernatant after centrifugation at 100 000 g) from female rat liver, suggesting a structural similarity between the cytoplasmic and the microsomal receptors.
Analysis of purified receptors by SDS electrophoresis and subsequent Western blot with 125I-labelled Mab as a probe showed one band corresponding to an Mr of 45 500 ± 2500 (n=5). The same band was obtained with 125I-labelled human GH, showing that the Mab binds to the unit which accommodates the hormone-binding site. The binding sites are, however, not identical since the binding of Mab to the receptor did not inhibit the hormone–receptor interaction. Whether this binding unit represents the intact receptor molecule or a part of it cannot be concluded.
Journal of Endocrinology (1989) 120, 401–407
